TB-500

Research Context

TB-500, in the form supplied by most research vendors, is a synthetic 43-residue peptide corresponding to the full-length sequence of endogenous Thymosin Beta-4 (Tβ4), an actin-binding protein present across mammalian tissues. The peptide is N-terminally acetylated and is supplied as a lyophilised white powder for laboratory investigation. Note that the name “TB-500” is also occasionally applied in the literature to a shorter 7-residue active fragment (Ac-LKKTETQ); this product is the full 43-residue analogue.

This material is supplied strictly for in-vitro laboratory investigation. Preclinical studies in the published literature have examined Thymosin Beta-4 in the context of G-actin sequestration assays, endothelial cell migration models, and dermal fibroblast culture. No therapeutic, prophylactic, or diagnostic application is claimed or implied, and the peptide is not approved for human or veterinary use in Australia.

Chemistry & Structural Modification

The supplied form is the acetylated 43-residue peptide with sequence Ac-SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES-OH. The central LKKTETQ motif (residues 17 to 23) is the canonical actin-binding region of the molecule.

• Molecular formula: C₂₁₂H₃₅₀N₅₆O₇₈S
• Molecular weight: 4963.4 g/mol
• CAS number: 77591-33-4; PubChem CID 16132341

Lyophilisation & Stability

Supplied as a sterile-filtered, lyophilised white powder under inert gas in a sealed vial. The freeze-dried form is the standard preservation state for research peptides of this molecular class and supports extended shelf life when stored as directed below.

Storage

Store lyophilised vial at 2 to 8°C, protected from light. (Long-term storage at −20°C is standard practice.)

Reconstituted solution: 2 to 8°C, used within 14 to 30 days. Avoid repeated freeze-thaw cycles.

Quality & Documentation

• Purity: ≥99% by HPLC
• Certificate of Analysis (COA): published on our COA page.